In the end, we found that strain MJM109 or the MJM109lacY mutant strain and the vector pGEMCP480 are the best choices for producing great quantities of dsRNA Resistance analyses and Northern blot showed that Tobacco mosaic virus infection could be inhibited by dsRNA, and the resistance was an RNAmediated virus resistance Satellite tobacco mosaic virus (STMV) is the spherical satellite to an obligatory rodshaped helper tobacco mosaic virus (TMV), which is required for replication STMV has 60 protein subunits of M (r) 17,500 on a T = 1 icosahedral capsid containing a singlestranded RNA genome of 1,059 bases STMV appears similar to another virus, STNV, but isThe RNAdependent RNA polymerase (replicase) mediating the replication of tobacco mosaic virus (TMV) has been investigated in a number of laboratories over a period of years Cellfree enzyme preparations have been prepared which can continue the synthesis of nascent complementary RNA, initiated in vivo;
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Double stranded rna is found in tobacco mosaic virus-However, the enzyme does not requireTobacco mosaic virus protein synthesis is correlated with doublestranded RNA synthesis and not singlestranded RNA synthesis Dawson WO The synthesis rates of three proteins of tobacco mosaic virus (TMV), 160, 110, and 175 kDa, were monitored at intervals after interruption of synthesis of TMV RNA
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Which virus has a double stranded RNA genome?5' TGGGCCCCTACCGGG 3'), which had been found to be Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family SolanaceaeThe infection causes characteristic patterns, such as "mosaic"like mottling and discoloration on the leaves (hence the name) TMV was the first virus to be
Tobacco Mosaic Virus is a ribovirus and contains single stranded RNA It was proved by the experiments of Frankel Conart that RNA is the genetic material in this virus It does not contain any DNA and is composed of 6% RNA surrounded by a hollow cylinder of portion subunits Double stranded RNA is found in Reovirus and Tumor virus Five major RNAs were isolated by polyacrylamide gel electrophoresis from purified virions of the yellow strain of cucumber mosaic virus (CMVY), althoA sucrose density gradientpurified, membranebound tobacco mosaic virus (tomato strain L) (TMVL) RNA polymerase containing endogenous RNA template was efficiently solubilized with sodium taurodeoxycholate Solubilization resulted in an increase in the synthesis of positivestrand, 64kb genomele
Which of the following relations will be found in the percentages of bases of a doublestranded DNA molecule? The Tomato leaf curl virus (ToLCV) is a bipartite (DNA) geminivirus whereas Cucumber mosaic virus (CMV) is a tripartite RNA virus, both infecting a wide range of host plants RNAi that is triggered by doublestranded RNA (dsRNA) molecules, is a powerful means to control plant viruses in transgenic plants, as well as in a nontransgenic manner in a process designatedBrief Review of Literature on Tobacco Mosaic Virus 2 Review of Literature on TMVRNÀ h Review of Literature on Infective RNA 5 MATERIALS AND METHODS 9 Preparation of Virus Stocks 9 Preparation of RNA 12 The Biological Assay System 15 Analytical Ultracentrifugation Techniques 17 Equipment and Techniques for Light Scattering Experiments 31
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Question 3 Double stranded RNA is found in A) Influenza Virus B) Reovirus C)Polio Virus D) T4 virus Answer B Question 4 Smallest virus is A) Foot and Mouth virus Question 47 Tobacco Mosaic Virus (TMV) genome is A) Double stranded RNA B) Single stranded RNA C) Polyribonucleotides D) ProteinaceousIt has been shown that many of the identified silencingsuppressor proteins bind long doublestranded RNA or siRNAs and thereby prevent assembly of the silencing effector complexes In this study, we show that the 122kDa replicase subunit (p122) of cruciferinfecting Tobacco mosaic virus (crTMV) is a potent silencingsuppressor protein The changes in doublestranded RNA content in tobacco leaves inoculated with tomato mosaic virus (ToMV) and its avirulent mutant N 14 were compared by 3 Huridine incorporation and resistance to RNase It was found that the content of doublestranded RNA (dsRNA) in leaves infected with N 14 was higher than in those infected with ToMV At the second
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Virusspecific RNA isolated from TMVinfected plants contains, in addition to genomic and subgenomiclength positivestrand RNA, genomiclength doublestranded RNA, designated replicativeform (RF) RNA and a heterogeneous family of RNAs, which are partly doublestranded and partly singlestranded, designated replica RNAi effects were induced in P citri, as demonstrated by specific target reductions of P citri actin, chitin synthase 1 and VATPase mRNAs after injection of the corresponding specific doublestranded RNA inducers We also used recombinant Tobacco mosaic virus (TMV) to express these RNAi effectors in Nicotiana benthamiana plantsThe infectious complete body of virus is called virion Virion is composed of nucleic acid and capid Therefore, virus is generally called nucleocapsid Viral Genomes The genomes of different viruses (sets of genes) may consist of doublestranded DNA, single stranded DNA doublestranded RNA, or single stranded RNA
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A nontransgenic approach involving the exogenous application of doublestranded RNA (dsRNA) has also been shown to induce resistance in tobacco against Cucumber mosaic virus (CMV) and Tobacco mosaic virus (TMV) In the present study, dsRNA molecules derived from the helper componentproteinase (HCPro) and coat protein (CP) genes of the ZYMV3 Double stranded RNA Double stranded RNA has been found within viral capsid in the reoviruses of animals and in the wound tumour virus and rice dwarf viruses of plants 4 Single stranded RNA Single stranded RNA is found in most of RNA viruses eg Tobacco mosaic virus, influenza virus, poliomylitis bacteriophage MS – 2, F – 2Either DNA or RNA but never both and can be either single stranded or double stranded Viruses non living pieces of genetic material surrounded by a capsid;
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Exogenous application of doublestranded RNA (dsRNA) in the tobaccoTobacco mosaic virus (TMV) pathosystem was shown previously to induce resistance against TMV providing an alternative approach to transgenesisIn the present study, we employed proteomics technology to elucidate the effect of TMV on tobacco as well as the effect of exogenous application of TMV p126 dsRNADoublestranded RNA It has been found within viral capsid in the reoviruses of animals and in the wound tumour virus and rice dwarf viruses of plants Viroid Small viruses without capsid Singlestranded RNA It is found in most of the RNA viruses eg tobacco mosaic virus, influenza virus, poliomyelitis, bacteriophage MS2, Avian leukemia virusThis changed in 1935 when the tobacco mosaic virus was crystallized and scientists demonstrated that the particles lacked any mechanisms necessary for metabolic function It was determined that viruses consisted of a nucleic acid, DNA or RNA, surrounded by a protein shell and the scientific view changed viruses are complex biochemical
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The tobacco mosaic virus, which plagues the plant but is harmless to humans, is hollowed out and filled with "small interfering RNA" molecules, or siRNA, which some scientists consider to be theNot cellular Tobacco Mosaic Virus has been shown to live how long outside of a host?Viral Morphology Viruses are noncellular, meaning they are biological entities that do not have a cellular structureThey therefore lack most of the components of cells, such as organelles, ribosomes, and the plasma membrane A virion consists of a nucleic acid core, an outer protein coating or capsid, and sometimes an outer envelope made of protein and phospholipid
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The 2 M LiClsoluble RNA fraction extracted from tobacco mosaic virus (TMV)infected tobacco plants contains, in addition to the viral replicative form of 4 x 10(6) MW, three smaller doublestranded (ds) RNA species with apparent molecular weights (estimated by polyacrylamide gel electrophoresis, using ds RNAs as markers) of 225, 11, and 023Virus T C U G A I 0 12 9 12 9 II 23 16 0 16 23Radioactive RNA probes were prepared which specifically hybridize with sequences complementary to 5' and 3' regions of tobacco mosaic virus (TMV) RNA These probes were used in Northern hybridization to locate TMVRNA minus strands in the subcellular fractions of infected tobacco protoplasts When the protoplasts were lysed with Triton X100
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Of the corresponding specific doublestranded RNA inducers We also used recombinant Tobacco mosaic virus (TMV) to express these RNAi effectors in Nicotiana benthamiana plants We found that P citri showed lower fecundity and pronounced death of crawlers after feeding on recombinant TMVinfected plants Taken together, our data show that actin,A strain of tobacco mosaic virus (TMV) named TMV Korean common strain has been isolated in Suwon (South Korea) from Nicotiana tabacum NC The fulllength double stranded cDNA of viral RNA has been synthesized and cloned Three oligonucleotide primers (5' ACTTAAGAATATTAGAAT 3';The changes in doublestranded RNA content in tobacco leaves inoculated with tomato mosaic virus (ToMV) and its avirulent mutant N14 were compared by 3Huridine incorporation and resistance to RNase It was found that the content of doublestranded RNA (dsRNA) in leaves infected with N14 was higher than in those infected with ToMV
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Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus in genus TobamovirusIt has a wide host range and its infection can cause severe damages and yield losses in many economically important crops (Scholthof et al 11)In its natural host tobacco and other closely related species, TMV can induce chlorosis, mosaic and necrosis in plant tissuesA/T = G/C What results would you expect if the Hershey and Chase experiment were conducted on tobacco mosaic virus? Keywords doublestranded RNA, plant proteomics, RNA interference, silencing suppressor, Tobacco mosaic virus, tobacco Citation Konakalla NC, Nitin M, Kaldis A, Masarapu H, Carpentier S and Voloudakis A (21) dsRNA Molecules From the Tobacco Mosaic Virus p126 Gene Counteract TMVInduced Proteome Changes at an Early Stage of Infection Front
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Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus species in the genus Tobamovirus that infects a wide range of plants, especially tobacco and other members of the family SolanaceaeThe infection causes characteristic patterns, such as "mosaic"like mottling and discoloration on the leaves (hence the name) TMV was the first virus to be discovered1 Virology 1969 Feb;37(2)276 Doublestranded tobacco mosaic virus RNA Ralph RK, Wojcik SJ PMID PubMed indexed for MEDLINE in HeLa cells infected with Vaccinia virus (a doublestranded ds DNA virus) 8 Furthermore, the polyadenylate tails were also found in Tobacco mosaic virus (TMV), Cucumber mosaic virus (CMV), Odontoglossum ringspot virus (ORSV), Cucumber green mottle mosaic virus (CGMMV), Tobacco
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Keywords Tobacco mosaic virus, Virus small interfering RNA, C2 domain ABArelated (CAR) gene Background Tobacco mosaic virus (TMV) is a positivesense singlestranded RNA virus in genus Tobamovirus It has a wide host range and its infection can cause severe damages and yield losses in many economically important crops (Scholthof et al 11)We have reported that doublestranded tobacco mosaic virus (TMV) RNA in cellfree extracts of TMVinfected leaves does not sediment with nuclei and chloro plasts centrifuged at 1000 g for 5 min How ever, 40% of the doublestranded TMV RNA in the extracts did sediment together with material sedimenting at 12,000 g for 5 minRabies virus is a () strand RNA virus that transcribes mRNAs from its genome (Group V) HIV1 is a singlestranded RNA retrovirus that uses reverse transcriptase to create a doublestranded DNA copy of its genome which is integrated into the host human's genome prior to
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Two species of doublestranded RNA (DS RNA) were isolated from TMVinfected leaf tissue with the aid of cellulose chromatography They have characteriContains either DNA or RNA which can be singlestranded or doublestranded nucleic acid surrounded by a protein coat (the capsid) The capsid is a protective proteinaceous coat that can be arranged in a helical pattern (like in tobacco mosaic virus, TMV) or a polygonal pattern (like the icosahedral patterns in adenovirus and bacteriophagePositivesense () strand can serve directly as mRNA to be translated to viral proteins, replication of RNA genome requires synthesis of the template () strand (complementary strand) to form a doublestranded RNA intermediate ex tobacco mosaic, coronavirus, flavivirus, polio
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Zucchini yellow mosaic virus (ZYMV) causes serious damage in a large number of cucurbits, and control measures are necessary Transgenic cucurbits expressing parts of the ZYMV genome have been shown to be resistant to the cognate virus A nontransgenic approach involving the exogenous application of doublestranded RNA (dsRNA) has also been shown to induceUp to 50 years African Cassave Mosaic Virus is found in africa, south america, south east asia
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Sinimung Plant Viruses Traditionally Viruses Are Named After The Most Conspicuous Symptom They Produce On The First Host A Virus Causing Mosaic On Tobacco Is Called Tobacco Mosaic Virus Whereas The Disease
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